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464 papers

Accuracy and Documentation of Richmond Agitation-Sedation Scale Assessments in a Cardiothoracic Critical Care Unit: A Prospective, Cross-Sectional Audit.

Szentgyorgyi L et al. · Jul 1, 2026

Background Sedation is a fundamental component of critical care and requires regular, accurate assessment to support its safe titration and minimise harm. International guidelines and local policy at the Wythenshawe Hospital's Cardiothoracic Critical Care Unit mandate hourly documentation of the Richmond Agitation-Sedation Scale (RASS). Aims This audit evaluated adherence to these standards and explored factors associated with documentation completeness and assessment accuracy. Study design A prospective, cross-sectional audit of bedside RASS assessment was conducted without prior notification. Nursing RASS scores were compared with expert auditor ratings. Data were collected using an anonymous, secure, auditor-completed online survey. Statistical analysis examined the relationship between documentation completion and RASS accuracy. Results Seventy-six nursing assessments were included. Most nurses were Band 5, with a median of 3.5 years of experience. Patients were predominantly male, mechanically ventilated and receiving sedation. Overall, 75% of nurse RASS assessments (n = 57) were accurate; however, only 33% (n = 25) were documented hourly as required by policy. Greater nursing experience was associated with improved accuracy in RASS. Deeper sedation was associated with lower agreement between nurse and expert assessments. Higher self-reported confidence was associated with poorer completion of documentation. Most discrepancies were small, with nurse and expert ratings differing by one RASS point. Conclusions This audit identified important gaps in the accuracy and documentation of nurse-led RASS assessment in a cardiothoracic critical care setting. The findings suggest that perceived familiarity and confidence may not be sufficient to ensure accurate and consistently documented sedation assessment. Relevance to clinical practice Suboptimal RASS documentation and scoring accuracy may limit reliable titration of sedation in critically ill patients and reduce the effectiveness of protocolised sedation strategies. These findings support targeted staff education, reinforcement of standardised assessment processes and improvements in documentation systems to strengthen sedation monitoring and patient safety.

Medicine

Paediatric Practitioners' Acceptance of the National Paediatric Early Warning System (PEWS).

Kaye LK et al. · Jul 1, 2026

Early detection of deterioration in children in hospital is essential for improving patient outcomes, prompting NHS England to introduce a digital National Paediatric Early Warning System (PEWS) in November 2023. Our service evaluation examined a locally delivered digital version of the PEWS system, introduced in July 2025 to explore paediatric staffs' acceptance before and after implementation, measured by the Technology Integration Evaluation Resource (TIER), based on the Technology Integration Model. Online surveys were completed pre- and post-implementation. Acceptance did not significantly change over time. However, user motivation, agency and perceived cost-benefit as well as perceptions of PEWS as an extension of self, were positively associated with acceptance, highlighting key psychological factors influencing digital health adoption.

Medicine

Antibiotics in Veterinary Ophthalmology: Resistance, Stewardship, and Emerging Antibiotic-Sparing Strategies.

Sebbag L et al. · Jul 1, 2026

Antimicrobial resistance (AMR) is a growing challenge in veterinary ophthalmology, particularly in cases of bacterial keratitis, where progressive stromal infection can threaten vision and globe integrity within hours to days. This review synthesizes current evidence on pathogen distribution, antimicrobial susceptibility profiles, multidrug resistance (MDR) prevalence, and determinants of nonsusceptibility in veterinary patients, highlighting the emerging role of antibiotic-sparing alternatives. Across contemporary studies, Staphylococcus pseudintermedius, β-hemolytic streptococci, and Pseudomonas aeruginosa are consistently among the most frequently isolated pathogens. The highest MDR burdens are reported in referral populations and among methicillin-resistant staphylococci worldwide. Feline data remain comparatively limited but show regional variability in resistance patterns, while equine studies reveal temporal shifts in isolate distribution and a rising prevalence of methicillin-resistant organisms in tertiary settings. Recent topical antimicrobial exposure is the most consistently identified predictor of reduced culture positivity and elevated resistance rates in subsequent ocular isolates, highlighting the importance of early microbiologic sampling and judicious antibiotic use. Interpreting antimicrobial susceptibility testing (AST) in ophthalmology remains challenging because clinical breakpoints are generally derived from systemic dosing regimens, despite substantially higher, albeit transient, drug concentrations being achieved at the ocular surface following topical administration. Moreover, the ocular surface microenvironment, including tear proteins, inflammation, biofilm formation, and concurrent serum therapy, may substantially influence antimicrobial activity and therapeutic response. The review concludes with practical ophthalmology-specific stewardship recommendations, a One Health perspective on resistant ocular pathogens, and a forward-looking discussion of antibiotic-sparing adjuncts within a broader multimodal strategy to preserve antimicrobial effectiveness.

Medicine

Bayesian Analysis of Postoperative Complication Risk Associated With Preoperative Exposure to Fine Particulate Matter: A Single-Center Cohort Study.

Pearson JF et al. · Jul 1, 2026

Background Air pollution, especially particle pollution, is increasingly recognized as a potential perioperative risk factor, yet modeling environmental exposures in surgical cohorts remains methodologically underdeveloped. We demonstrate a Bayesian hierarchical framework to quantify probabilistic associations between preoperative fine particulate matter (PM 2.5 ) exposure and postoperative complications, highlighting its interpretability and flexibility for clinical environmental epidemiology. Methods We conducted a single center, retrospective cohort study using data from 49,615 surgical patients in Utah who underwent elective surgical procedures from 2016 to 2018. Patients' addresses were geocoded and linked to daily Census-tract level PM 2.5 estimates. The exposure variable was defined as the maximum PM 2.5 concentrations in the 7 days prior to surgery. The binary outcome was a composite of postoperative complications: pneumonia, surgical site infection, urinary tract infection, sepsis, stroke, myocardial infarction, or thromboembolic event. A hierarchical Bayesians regression model with weakly informative priors was used adjusting for age, sex, season, neighborhood disadvantage, and the Elixhauser index of comorbidities with census tract as a group (random) effect. We present posterior estimates with credible intervals, highlight model transparency and sensitivity, and discuss contrasts with standard frequentist methods. Results Postoperative complications were associated in a dose-dependent manner with higher concentrations of PM 2.5 exposure. We found a relative increase of 8.2% in the odds of complications (OR = 1.082) for every 10.ug/m 3 increase in the highest single-day 24-h PM 2.5 exposure during the 7 days prior to surgery. For an increase in PM 2.5 from 1 to 30 ug/m 3 , the odds of complication rose to over 27% (95% CI: 4%-55%). The results were robust across prior choices and model specifications. We report full posterior distributions and highlight advantages of Bayesian modeling for uncertainty quantification and clinical interpretability. Conclusions This case study demonstrates the application of hierarchical Bayesian modeling to quantify the probabilistic associations between preoperative PM 2.5 exposure and postoperative complications, highlighting transparent risk estimation and uncertainty characterization that may inform the design of future multicenter perioperative environmental studies. Editorial comment Using Bayesian statistical analysis, the authors demonstrate a dose-dependent risk for postoperative complications in patients exposed to air polluted with fine particulate matter with a size of less than 2.5 μm.

Environmental Science

Helping families thrive while developing a strong working alliance: The benefits of Recipe 4 Success.

Braaten SM et al. · Jul 1, 2026

This study explored whether the strength of parent-home visitor working alliances might alter the benefits of Recipe 4 Success, a highly structured food-based curriculum designed to promote parents' sensitive scaffolding, responsive food parenting practices, toddlers' self-regulation, and healthy eating habits. This study included 242 parents and their toddlers residing in the United States, most of whom were living in poverty (37% white, 25% Black, 19% Latiné, 17% Multiracial, and 2% Asian; median income = $1555 per month). Families were randomly assigned to Recipe 4 Success or usual practice home visits within home visitor caseload. Home visitors tended to rate their working alliance as very strong versus less strong. Within-group regression equations revealed that, among families with a less strong working alliance, Recipe 4 Success was more effective than usual practice home visits in improving parents' sensitive scaffolding, responsive food parenting practices, and toddlers' self-regulation. In contrast, among families who had a strong working alliance, Recipe 4 Success was more effective than usual practice home visits in changing toddlers' healthy eating habits, including reducing body mass index for toddlers with overweight/obesity. These findings highlight how families with varying strengths of working alliances may benefit differently from highly structured evidence-based curricula.

Medicine

Prevalence, Disease Onset and Clinical Outcome in Arginase 1 Deficiency: Cross-Border Surveillance in Germany, Austria, and Switzerland.

Scharre S et al. · Jul 1, 2026

Arginase 1 deficiency (ARG1-D) is an ultra-rare urea cycle disorder characterized by progressive spastic paraplegia, developmental delay, epilepsy, and episodic hyperammonemia. Evidence on prevalence and clinical presentation is scarce. Therefore, epidemiology and the phenotypical spectrum were assessed in Germany, Austria, and Switzerland (DACH region). We conducted a questionnaire-based, cross-sectional study of confirmed ARG1-D patients in the DACH region. Patients were stratified into early-diagnosed (newborn screening [NBS] or high-risk family screening [HR]) and diagnosed after symptom onset. We evaluated clinical, biochemical, and therapeutic characteristics of individuals with confirmed ARG1-D. Epidemiological prevalence estimates were derived using national population data. A total of 20 patients were identified (Germany: 12, Austria: 7, Switzerland: 1). Eight were diagnosed early (NBS: 4, HR: 4) and 12 after symptom onset. Symptomatically diagnosed patients (median age 11 years) presented with a broad range of clinical manifestations, specifically progressive spastic paraplegia (67%), epilepsy (58%), dystonia (46%), developmental delay (58%), and hepatopathy (50%). Median age at diagnosis was 35 months in symptomatic patients versus 1 month in early-diagnosed patients (p = 0.03). Estimated pediatric prevalence was 1:1042080 in the DACH region, with high regional differences. Hyperammonemia was reported in 72%. Enzyme therapy had been initiated in 21%; 2 patients underwent liver transplantation. ARG1-D is a rare disease with a prevalence of approximately 1:1000000 individuals, and a complex and progressive clinical phenotype. Detection via NBS or HR allows early diagnosis and treatment initiation, potentially altering the clinical outcome.

Biochemistry, Genetics and Molecular Biology

Mental Health Outcome Trends in a Nationally Representative Sample of Canadian Migrant Adolescents From 2014 to 2022.

Ji D et al. · Jul 1, 2026

Background Migrant youth are at disproportionate risk of mental health challenges. Overcoming barriers to accessing services requires large-scale data to inform policies and interventions. This study maps mental health outcome trends of migrant youth over 8 years. Methods Canadian Health Behavior in School-aged Children study data from 2014, 2018, and 2022 were analyzed for mental health outcome trends. Age-adjusted logistic regressions examined health across years stratified by migrant status, separately by sex. Using nonmigrants as the referent group and 2014 as the referent year, contrasts for disparities were examined across migrant status to test widening, narrowing, or stability in differences of outcome prevalences between migrant and nonmigrant youth. Results Health worsened from 2014 to 2022 among migrants, especially migrant girls. Compared to 2014, life satisfaction, health, and self-confidence for migrant youth dropped in 2022. Health complaints and feeling sad/hopeless increased in 2022 among girls. Migrant youth reported fewer health complaints than nonmigrants in 2018 and 2022. Implications Investment in free/affordable, culturally safe/relevant, confidential school-based mental health supports with avenues for community collaboration are recommended. Conclusions Mental health outcomes worsened from 2014 to 2022, especially among migrants and girls; however, migrant youth exhibit resilience to adversity.

Psychology

Oromucosal Administration of a Cannabidiol-Enriched Cannabis sp. Extract for 2 Weeks Moderately Reduces Cold Hyperalgesia in Rats With Neuropathic Pain.

Campos RMP et al. · Jul 1, 2026

Background Chronic neuropathic pain (CNP) involves complex interactions between resident and peripheral immune cells, as well as modulation of the endocannabinoid system. Cannabis-based products have emerged as promising therapeutic options, but their effects on immune cells remain unclear. This study evaluated the effects of a cannabidiol (CBD)-enriched Cannabis sp. extract administered via the oral mucosa in a CNP model. Methods Two-month-old Wistar rats of both sexes were randomly assigned to four groups. Two groups underwent chronic constriction injury (CCI) of the sciatic nerve, while the remaining groups underwent sham surgery (SHAM). Starting at Day 7 post-CCI, rats received either the CBD-enriched extract (10 mg/kg) or vehicle for 15 days. Mechanical and thermal sensitivity were assessed using the von Frey, acetone and hot plate tests. Ipsilateral spinal cord segments were analysed by flow cytometry, immunohistochemistry and western blotting. Results CCI induced thermal hyperalgesia and mechanical allodynia. Treatment with the CBD-enriched extract modestly reduced cold sensitivity in CCI rats of both sexes but did not improve heat sensitivity and had a significant but minor effect on mechanical thresholds. CCI increased spinal cord microglial cell numbers, particularly in females, an effect not modulated by treatment. No significant changes were detected in immune cell infiltration or CB1R, CB2R, or TRPV1 protein expression across groups. Conclusion Oromucosal treatment with a CBD-enriched extract modestly alleviates cold hypersensitivity in both sexes. Neuroimmune alterations in the spinal cord at 22 days post-CCI indicate a predominance of microglia, with no detectable peripheral immune cell infiltration, regardless of treatment. Significance statement This manuscript contributes to addressing gaps in the literature regarding preclinical models evaluating Cannabis sp. based products using treatment protocols that more closely reflect patient use, as commercially available cannabidiol-enriched extract and the oromucosal route, consistent with the route commonly used by patients. In addition, all experiments were conducted in animals of both sexes, addressing a well-recognised limitation of many preclinical studies in chronic pain research. Notably, the International Association for the Study of Pain has emphasised the need for further research in this area before endorsing the use of Cannabis sp. based products for the treatment of chronic pain.

Medicine

Lean, Non-Autoimmune Young-Onset Diabetes in Bangladesh: A Metabolically Obese Phenotype With Disproportionate Insulin Secretory Defect.

Hasan M et al. · Jul 1, 2026

Background Young-onset diabetes in South Asians frequently manifests in lean individuals, but the metabolic drivers of this phenotype remain poorlycharacterised. Objective This study aimed to evaluate the clinical, biochemical, and insulin-related indices of lean, non-autoimmune young-onset diabetes mellitus (DM) among Bangladeshi young adults. Methods This comparative cross-sectional study (2023-2024) enrolled 373 participants (aged 18-34 years) categorised into four groups based on glycemic status and body mass index (BMI): Lean DM (n = 53), obese DM (n = 125), lean non-DM (n = 78), and obese non-DM (n = 117) from the Young-diabetes Clinic of the Department of Endocrinology, Bangladesh Medical University, Dhaka after excluding those with positive islet autoantibodies, low C-peptide, pancreatic pathology, and monogenic variants. β-cell function (HOMA2-B) and insulin resistance (HOMA2-IR) were quantified using the HOMA2 C-peptide calculator. Results Lean DM participants were comparable to other groups in age, sex, and family history, though smoking was more prevalent than in lean controls (p  Conclusion Lean young-onset diabetes in Bangladesh appears to be a phenotype of disproportionate insulin secretory defect with only modest insulin resistance and metabolic dysfunction. These features highlight the need for targeted screening and individualised management.

Biochemistry, Genetics and Molecular Biology

Crossing Borders of Care: The Professionalization of Women in Nursing and China-US Collaboration at Xiangya, 1909-1926.

Tang Y et al. · Jul 1, 2026

This article examines the development of modern nursing education in China through a case study of the Xiangya School of Nursing in Changsha between 1909 and 1926. Founded in 1911 by the Yale-in-China Association, a non-denominational mission, Xiangya was among the earliest nursing schools in China to promote undergraduate nursing education and public health nursing. Drawing on archival materials, published primary sources, and Chinese and English historiography, we analyze the cultural, political, and gendered challenges that shaped the school's development under the leadership of American nurse Nina Gage. While Gage initially sought to implement an Americanized model of nursing education, she and her colleagues adapted admission policies, educational goals, and curricula to local social and cultural conditions. Recognizing the need for Chinese nursing leadership, they prioritized the establishment of a Bachelor of Science degree to prepare nurses for teaching, administration, and public health roles. By 1926, Xiangya had trained approximately 16% of China's qualified nurses and had become one of only two schools in China offering university-level nursing education. This history highlights the interplay of transnational influence, cultural adaptation, and gendered reform in the professionalization of nursing in early 20th-century China.

Nursing